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目的通过对系统性红斑狼疮(SLE)患者外周血淋巴细胞(PBL)早期凋亡率测定,探讨其在SLE发病中的作用及相关性。方法应用最新的AnnexinⅤ试剂盒及流式细胞仪对SLE患者及年龄、性别相匹配的正常人PBL进行早期凋亡细胞的检测;并运用免疫印迹法检测SLE患者组自身抗体谱及应用免疫浊度法检测补体C3含量。结果SLE活动期患者PBL早期凋亡率(13.46±4.13)明显高于稳定期患者(5.29±2.74)及正常人对照(5.14±2.33);稳定期患者与正常人对照差异无显著性(P>0.05);活动期患者中初发病组与已用皮质类固醇或免疫抑制剂治疗组差异无显著性(P>0.05)。SLE患者中,能测定出两种以上自身抗体阳性者凋亡率(13.44±4.40)明显高于至多有一种自身抗体阳性者凋亡率(6.72±3.86);且活动期患者PBL凋亡率与补体C3含量呈高度负相关(r=-0.86,P<0.01)。结论SLE患者PBL凋亡率明显高于正常人,可能通过释放过量自身抗原的方式诱导一系列自身抗体的产生,从而参与SLE的发生。
Objective To investigate the early apoptosis rate of peripheral blood lymphocytes (PBL) in patients with systemic lupus erythematosus (SLE) and explore its role in the pathogenesis of SLE. Methods The latest Annexin V kit and flow cytometry were used to detect the early apoptotic cells in SLE patients and PBLs of normal age and sex. The autoantibodies of SLE patients were detected by immunoblotting and immunoturbidimetry Act detection of complement C3 content. Results The early apoptosis rate of PBL in active SLE patients (13.46 ± 4.13) was significantly higher than that in stable patients (5.29 ± 2.74) and normal controls (5.14 ± 2.33). There was no significant difference between stable patients and normal controls (P> 0.05). There was no significant difference between the initial onset group and the corticosteroid or immunosuppressive group in the active stage (P> 0.05). In SLE patients, the apoptotic rates of two autoantibodies positive (13.44 ± 4.40) were significantly higher than those of at least one autoantibody positive (6.72 ± 3.86) Complement C3 content was highly negatively correlated (r = -0.86, P <0.01). Conclusion The apoptosis rate of PBL in patients with SLE is significantly higher than that in normal people, which may induce the production of a series of autoantibodies through the release of excessive autoantigen to participate in the pathogenesis of SLE.