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将BALB/c(H 2 d)小鼠及C5 7BL/ 6 (H 2 b,B6 )小鼠的脾细胞分别经尾静脉注射给对方 ,2d后再分别腹腔注射环磷酰胺 (CY ) 15 0mg/kg ,CY注射后 1d对BALB/c及B6小鼠进行联体 (parabiosis) ,1周后分开并进行相互间的植皮。发现BALB/c小鼠对B6小鼠的皮肤耐受期明显延长 (MST =2 5 7d ) ,但是B6对BALB/c小鼠的皮肤耐受期无明显延长 (MST =11 9d )。利用流式细胞仪分析技术 (FACS )分别对上述处理的BALB/c及B6小鼠在联体分开后的第 1天和第 30天进行胸腺及脾脏嵌合程度的检查 ,发现皮肤耐受期与嵌合程度不呈正相关。对联体后耐受的BALB/c小鼠进行体内和体外细胞转移实验 ,均未显示耐受小鼠脾细胞中存在抑制细胞活性。在耐受BALB/c→B6小鼠的单向混合淋巴细胞反应 (MLR )体系中加入外源IL 2可以部分反转耐受BALB/c小鼠脾细胞的增殖反应 ,表明本实验诱导耐受的机制可能与克隆不应答 (clonalanergy )有关。
Spleen cells of BALB / c (H 2 d) mice and C5 7BL / 6 (H 2 b, B 6) mice were respectively injected into the tail vein. Two days later, intraperitoneal injection of cyclophosphamide (CY) / kg, BALB / c and B6 mice were parabiosis 1 day after CY injection, separated one week later and grafted to each other. BALB / c mice were found to have significantly prolonged skin endurance (MST = 257 days) in B6 mice, but no significant prolongation of BALB / c mice skin tolerance (MST = 11 9 days). The degree of chimerism in the thymus and spleen of BALB / c and B6 mice treated above was examined by flow cytometry (FACS) on day 1 and day 30, respectively, and found that the skin endurance period There was no positive correlation with chimerism. In vivo and in vitro cell transfer assays of post-mortem BALB / c mice did not show the presence of inhibitory cell activity in tolerized mouse splenocytes. Addition of exogenous IL2 to a one-way mixed lymphocyte reaction (MLR) system that tolerated BALB / c → B6 mice partially reversed the proliferative response to splenocytes from BALB / c mice, indicating that this experiment induced tolerance The mechanism may be related to clonalanergy.