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目的:通过对新发现未经治疗的HIV感染者的外周血淋巴细胞亚群分布调查,了解HIV感染者的免疫状况,估计其疾病进展情况。方法:收集294名新发现HIV感染者的抗凝全血,用流式细胞仪、三色荧光抗体试剂和计数微球检测CD3+、CD3+CD4+和CD3+CD8+淋巴细胞绝对值,计算CD4+/CD8+的比值。用SPSS12.0软件分析数据。结果:淋巴细胞亚群检测结果均值:CD3+为1218±608个/μl,CD3+CD4+为303±232个/μl,CD3+CD8+为811±421个/μl,CD4+/CD8+为0.41±0.31。男性和女性的CD4+/CD8+比值有差异,表现为女性高于男性。不同年龄组的CD3+和CD3+CD4+绝对计数及CD4+/CD8+比值均有差异,随着年龄的增长其数值均呈下降趋势。不同传播途径分组在淋巴细胞亚群分布结果上无显著差异。结论:HIV感染者已经进入发病高峰期,应对其定期监测CD3+CD4+和其它淋巴细胞亚群数量,以便估计疾病进展情况和把握治疗时机。
OBJECTIVE: To investigate the distribution of peripheral blood lymphocyte subsets in newly discovered untreated HIV-infected individuals and to understand the immunological status of HIV-infected individuals and to assess their disease progression. METHODS: A total of 294 anticoagulated whole blood samples from newly infected HIV-infected individuals were collected. The absolute values of CD3 +, CD3 +, CD4 + and CD3 + CD8 + lymphocytes were measured by flow cytometry, trichrome fluorescent antibody reagent and counting microspheres. CD4 + / CD8 + The ratio. Analyze the data with SPSS12.0 software. Results: The mean values of lymphocyte subsets were 1218 ± 608 cells / μl for CD3 +, 303 ± 232 cells / μl for CD3 + CD4 +, 811 ± 421 cells / μl for CD3 + CD8 +, and 0.41 ± 0.31 for CD4 + / CD8 +. There is a difference in the ratio of CD4 + / CD8 + between men and women, showing that women are higher than men. The absolute counts of CD3 + and CD3 + CD4 + and the ratio of CD4 + / CD8 + in different age groups were different. The values decreased with age. There was no significant difference in the distribution of lymphocyte subgroups among the different routes of transmission. CONCLUSIONS: HIV-infected individuals have entered the peak incidence period and should regularly monitor CD3 + CD4 + and other lymphocyte subsets in order to assess disease progression and timing of treatment.