菌种分离培养的几点体会

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菌种是食用菌栽培成败的关键,要获得理想效果,必须选用优良菌种。培养基物选择食用菌分离培养的基物很多,概括起来有三种:完全培养基-能满足任何微生物的营养需要,其成分都是已知的,如察氏、马丁培养基;基本培养基-由天然原料与部分已知有机物合成,如马铃薯、葡萄糖、琼脂培养基(简称PDA);补充培养基-菌株经诱变后在培养基中补充某种有机物质和碳源、氨源、无机盐、生长素等。目前分离培 Strain is the key to the success or failure of edible fungi cultivation, to obtain the desired effect, we must choose good strains. Medium selection Edible mushroom isolation and culture of a large number of substrates, summed up in three ways: Complete medium - to meet the nutritional needs of any microorganism, the composition is known, such as Czochralski, Martin medium; basic medium - It is synthesized from natural raw materials and some known organic compounds, such as potato, glucose, agar medium (PDA); supplemented medium - strain after mutagenesis in the medium to add some organic matter and carbon source, ammonia source, inorganic salts , Growth hormone and so on. The current separation training
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