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目的 研究神经节苷脂GM1对新生鼠缺血缺氧性脑损伤的保护作用及HSP70 表达的影响。方法 建立新生鼠缺氧缺血性脑病动物模型 ,应用组织化学和免疫组织化学法观察缺血缺氧后 3h、6h、1d、3d、7d、14d脑组织的病理变化及HSP70 的表达 ,以及GM1给药后的变化。结果 单纯缺血缺氧组 ,在缺血缺氧后 3h缺血侧皮层、海马CA3 区、纹状体开始有少量HSP70 表达 ,2 4h达高峰 ,14d未见HSP70 表达 ;GM1给药组 ,脑组织损伤明显减轻 ,6h才开始有少量HSP70 表达 ,7d未见有表达。结论 GM1对新生鼠缺血缺氧性脑损伤具有明显的保护作用 ;HSP70 的诱导表达是缺血缺氧性脑损伤敏感而可靠的指标 ,GM1可抑制这种表达。
Objective To investigate the protective effect of ganglioside GM1 on hypoxic-ischemic brain damage in neonatal rats and the effect of HSP70 on it. Methods The animal model of neonatal hypoxic-ischemic encephalopathy was established. The histopathological changes and the expression of HSP70 were observed at 3h, 6h, 1d, 3d, 7d, 14d after hypoxia-ischemia by histochemistry and immunohistochemistry. Changes after administration. Results In the hypoxia-ischemia group, a small amount of HSP70 began to be expressed in the ischemic cortex, hippocampal CA3 region and striatum 3h after hypoxia-ischemia, peaked at 24 hours and no HSP70 expression at 14th day. Tissue damage significantly reduced, 6h began to have a small amount of HSP70 expression, 7d no expression. Conclusion GM1 has a protective effect on hypoxic-ischemic brain damage in neonatal rats. Induced expression of HSP70 is a sensitive and reliable indicator of hypoxic-ischemic brain damage. GM1 can inhibit this expression.