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以大蒜花序轴为试材,采用植物组织离体培养方法,研究了不同激素、蔗糖浓度及培养基的种类对花序轴分化及继代培养的影响。结果表明:以MS为基本培养基诱导大蒜花序轴分化的最佳培养条件为MS+6-BA 1.5mg/L+NAA 0.1mg/L,30g/L蔗糖,pH 5.8,分化率可达100%,增殖系数达46.3;最佳的继代培养基为1/2MS+6-BA 0.1mg/L+NAA 0.5mg/L,成活率可达100%,且长势最强。
The method of plant tissue culture in vitro was used to study the effects of different hormones, sucrose concentrations and media on the differentiation and subculture of inflorescences. The results showed that MS medium supplemented with 1.5 mg / L NAA, 0.1 mg / L NAA, 30 g / L sucrose, pH 5.8, and the optimal culture conditions were 100% , The proliferation coefficient of 46.3; the best subculture medium is 1 / 2MS + 6-BA 0.1mg / L + NAA 0.5mg / L, the survival rate of up to 100%, and the strongest growth.