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目的 探讨儿童急性肾小球肾炎与血管紧张素转换酶 (ACE)基因多态性的关系。方法 用PCR方法检测了 2 0 0 0～ 2 0 0 3年长春市儿童医院 6 6例儿童急性肾小球肾炎患儿ACE基因多态性 ,探讨ACE基因多态性与尿蛋白量、血尿、高血压、氮质血症的关系。并以 5 0名健康儿童ACE基因型作对照。结果 急性肾小球肾炎患儿ACE DD基因频率为 0 12 ,ACE II基因频率为 0 5 6 ,ACE DI基因频率为 0 32 ,急性肾小球肾炎患儿ACE基因多态性与正常对照组存在明显差异 ;ACE II基因型与肾小球肾炎的遗传易感性相关联 ,OR =2 4 77;急性肾小球肾炎ACE基因型与血尿、高血压、氮质血征有明显相关性 ,出现血尿、高血压、氮质血症的急性肾小球肾炎患者ACE基因型多为II型 ,P <0 0 1。结论 ACE II基因可能是发生急性肾小球肾炎的危险因子。检测儿童ACE基因 ,对于早期发现和及时防治儿童急性肾小球肾炎有重要意义
Objective To investigate the relationship between acute glomerulonephritis and angiotensin-converting enzyme (ACE) gene polymorphism in children. Methods The PCR method was used to detect the polymorphism of ACE gene in 66 children with acute glomerulonephritis in Children’s Hospital of Changchun City from 2000 to 2003. The correlation between ACE gene polymorphism and urinary protein, hematuria, Hypertension, azotemia relationship. Fifty healthy children with ACE genotype control. Results The frequency of ACE DD gene in children with acute glomerulonephritis was 0 12, the frequency of ACE II gene was 0 5 6, the frequency of ACE DI gene was 0 32. The polymorphism of ACE gene in children with acute glomerulonephritis was significantly higher than that in normal controls Significantly different; ACE II genotype and genetic susceptibility to glomerulonephritis associated OR = 2 4 77; acute glomerulonephritis ACE genotype and hematuria, hypertension, blood nitrogen was significantly related to the presence of hematuria The ACE genotypes in patients with acute glomerulonephritis of hypertension and azotemia were mostly type II, P <0.01. Conclusion ACE II gene may be a risk factor for acute glomerulonephritis. Detection of ACE gene in children, for early detection and timely prevention and treatment of acute glomerulonephritis in children is of great significance