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目的:尝试将基于保留机制法的反相色谱柱选择策略应用到中药的多成分分析中,并通过实验考察其可行性,为提高法定标准在不同实验室之间的重现性提供依据。方法:在2台不同的高效液相色谱仪和40根不同品牌、型号的C18色谱柱上,采集大黄、补骨脂中多种成分的液相色谱,对大黄中9种成分、补骨脂中11种成分的分离效果进行评价,尤其是对关键峰对的分析,借助USP法(United States Pharmacopeial approach,USP approach)和PQRI法(PQRI approach)2种等效色谱柱选择方法,比较不同色谱柱对分离的影响。结果:相似度高的色谱柱对大黄的分离效果接近,对补骨脂的分离效果规律性不明显。结论:色谱柱对中药成分的分离效果不仅与色谱柱本身的性质有关,与待测成分的性质也相关,因此目前的色谱柱选择系统尚无法解释所有问题。但是相对于随机选择色谱柱,采用这些方法选择色谱柱仍可以提高方法的重现性;结合基于某种中药或某类成分的色谱柱数据库,建立色谱柱选择系统是可行的。
OBJECTIVE: To try to apply the strategy of selecting column based on retention mechanism to the multi-component analysis of traditional Chinese medicine (TCM). The feasibility of this method was also investigated experimentally to provide basis for improving the reproducibility of statutory standards in different laboratories. Methods: Liquid chromatography was used to collect the constituents of rhubarb and psoralen in two different HPLC and 40 C18 columns of different brands and types. The contents of nine components in rhubarb, psoralen , Especially in the analysis of key peak pairs. Two kinds of equivalent column selection methods, such as the USP approach and the PQRI approach, were used to compare different chromatograms Effect of column on separation. Results: The high similarity column was close to the separation effect of rhubarb, and the regularity of separation effect on psoralen was not obvious. Conclusion: The separation of traditional Chinese medicine from the column is not only related to the nature of the column, but also to the nature of the components to be tested. Therefore, the current system for column selection still can not explain all the problems. However, compared with random selection of columns, the selection of columns by these methods can still improve the reproducibility of the method. It is feasible to establish a column selection system based on a traditional Chinese medicine or a certain type of column database.