论文部分内容阅读
目的 :用贝氏柯克斯体感染小鼠 ,观察引起的病理学变化并检测贝氏柯克斯体的抗原分布和DNA在靶细胞中的表达 ,探讨Q热病变规律并建立特异性诊断方法。方法 :腹腔注射贝氏柯克斯体悬液感染BALB/c小鼠 ,发病后解剖 ,观察各脏器的主要病变 ;做脾触片染色后检查 ;取各脏器制作石蜡切片 ,于光镜下观察 ;应用免疫组化染色显示贝氏柯克斯体的抗原分布 ;利用原位杂交从分子水平检测贝氏柯克斯体。结果 :剖检可见肝脏、脾脏有明显病变 ;脾触片染色后在油镜下可查见红色短杆状物质 ;光镜下肝脏、脾脏有非典型肉芽肿形成 ,肺多呈间质性肺炎 ,脑神经胶质细胞增生 ;免疫组化和原位杂交阳性信号多位于单核 巨噬细胞系统胞浆内。结论 :通过光镜观察感染贝氏柯克斯体小鼠发生的病理学变化 ,主要可见肝脏、脾脏有非典型肉芽肿 ,肺呈间质性肺炎 ,脑神经胶质细胞增生 ,其他脏器无明显变化 ;采用免疫组化染色和原位杂交 ,分别从抗原、基因水平原位检测贝氏柯克斯体 ,显示阳性信号多位于单核 巨噬细胞系统胞浆内 ,建立了特异性的Q热诊断方法。
OBJECTIVE: To infect mice with Bemisia crassavirus, to observe the pathological changes induced and to detect the distribution of antigens and the expression of DNA in target cells of Bemisia cocos, explore the rules of Q fever and to establish a specific diagnostic method . Methods: BALB / c mice were inoculated intraperitoneally with Behcet’s Kirby’s suspension and dissected after the onset of disease to observe the main pathological changes of the organs. Spleen contacts were stained and examined. Paraffin sections were made from each organ. The immunohistochemistry was used to detect the antigenic distribution of Beauveria bassiana. The in situ hybridization was used to detect Bemisia cercis from the molecular level. Results: The liver and spleen of the spleen were obviously lesioned by autopsy. The red short rod-like substance could be seen under the oil spleen after stained by the spleen contact lens. Atypical granuloma formed in the liver and spleen under the light microscope. Most of the lungs were interstitial pneumonia , Glial cell proliferation; immunohistochemistry and in situ hybridization positive signals are located in the cytoplasm of mononuclear macrophage system. CONCLUSION: The pathological changes in mice infected with C. bachii were observed by light microscopy. The main pathological changes of the infected mice were observed atypical granulomas in liver and spleen, interstitial pneumonia in lungs, glial cell proliferation in other organs Significant changes were detected by immunohistochemical staining and in situ hybridization, respectively, from the antigen, gene level in situ detection of C. cynocephala Kesi body showed positive signals were located in the cytoplasm of mononuclear macrophage system to establish a specific Q Thermal diagnostic methods.