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给ＲＦ／Ｊ品系小鼠注射ＭＮＵ（Ｍｅｔｈｙｌｎｉｔｒｏｓｏｕｒｅａ）３０ｍｇ／ｋｇ／周，共５周，全部小鼠都发生Ｔ细胞淋巴瘤。为了槁清楚其染色体变化及意义，我们仔细研究了其原发性淋巴瘤及其在裸小鼠（ｎｕｄｅｍｉｃｅ）和同基因小鼠（ＲＦ／Ｊ）体内连续传代培养的细胞染色体。结果表明，早期的绝大多数变异是染色体数目异常，少数为结构变化。虽然在全部小鼠中受累的染色体不完全相同，但常常局限在某些染色体上。胸腺瘤及其传代细胞的染色体畸变皆含有第１５、１４、３、１２号及Ｘ染色体，说明这些染色体含有促进肿瘤多步发生的基因，即癌基因（ｏｒｉｃｏｇｅｎｅ）及抑癌基因（ｔｕｍｏｒｓｕｐｐｒｅｓｓｏｒｇｅｎｅ）。ＭＮＵ的高度组织特异性和明显的致癌作用，很可能是其对Ｔ细胞染色体变化的优先转化作用所致。
RF/J strain mice were injected with MNU (Methylnitrosourea) at 30 mg/kg/week for 5 weeks, and all mice developed T-cell lymphoma. In order to understand its chromosome changes and its significance, we carefully studied its primary lymphoma and its cell chromosomes that were continuously subcultured in nudemice and syngeneic mice (RF/J). The results showed that most of the early mutations were abnormal chromosome numbers, and a few were structural changes. Although the chromosomes involved in all mice are not identical, they are often confined to certain chromosomes. The chromosomal aberrations of thymoma and its passaged cells all contain No. 15, 14, 3, 12 and X chromosomes, indicating that these chromosomes contain genes that promote multistep tumorigenesis, namely oncogenes and tumor suppressor genes. The high tissue specificity and apparent carcinogenicity of MNU is likely due to its preferential transformation of chromosome changes in T cells.