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目的 :研究GE7导入系统体内导入效率及介导抑癌基因NOEY2体内导入后对人上皮性卵巢癌裸鼠网膜移植瘤的治疗作用。方法 :将人上皮性卵巢癌细胞株Hey细胞种植于裸鼠皮下成瘤后半包埋缝于裸鼠脾区网膜建立网膜移植瘤模型。将GE7- β -gal四元复合体经腹腔注入荷瘤鼠体内用X -gal染色法检测基因导入效率 ,同法导入生理盐水、GE7-pcDNA3四元复合体及GE7-pcDNA3-NOEY2四元复合体 ,研究其抑制肿瘤生长的作用。结果 :人上皮性卵巢癌裸鼠网膜移植瘤的成瘤率达 10 0 % ,3周后瘤体达 3 68± 0 82g。GE7- β -gal四元复合体经腹腔注入荷瘤鼠体内 12h后 β -gal即有表达 ,4 8h后以瘤体、肝、脾表达率最高。GE7-pcDNA3-NOEY2四元复合体腹腔注射 3周后瘤体生长抑制率为 4 0 81% (P <0 0 5)。结论 :人上皮性卵巢癌裸鼠网膜移植瘤模型具有实用性。GE7导入系统体内导入基因效率高 ,具有相对肿瘤靶向性。GE7导入系统介导抑癌基因NOEY2体内导入后能有效抑制上皮性卵巢癌的生长。
OBJECTIVE: To study the in vivo efficiency of GE7-mediated delivery system and the therapeutic effect of NOEY2 in vivo on the transplanted human omental epithelial ovarian tumor in nude mice. Methods: Hey cells of human epithelial ovarian cancer cell line were implanted subcutaneously in nude mice to establish an omentum xenograft tumor model. The GE7- β-gal quaternary complex was injected into tumor-bearing mice intraperitoneally to detect the efficiency of gene introduction by X-gal staining. The same method was used to introduce NS, GE7-pcDNA3 quaternary complex and GE7-pcDNA3-NOEY2 quaternary complex Body, to study its role in inhibiting tumor growth. Results: The tumorigenic rate of human ovarian epithelial ovarian cancer omentum transplanted in nude mice reached 10%. The tumor size reached 3 68 ± 0 82g after 3 weeks. After intraperitoneal injection of GE7- β -gal quaternary complex into the tumor-bearing mice for 12h, the expression of β-gal was observed. After 48h, the expression rate of tumor, liver and spleen was the highest. The growth inhibition rate of GE7-pcDNA3-NOEY2 quaternary complex after 3 weeks of intraperitoneal injection was 40 81% (P <0 05). Conclusion: The human ovarian epithelial ovarian cancer omentum transplantation model has practicality. GE7 introduction system in vivo gene import efficiency, with relative tumor targeting. GE7-mediated system-mediated suppression of gene NOEY2 in vivo can effectively inhibit the growth of epithelial ovarian cancer.