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目的:观察黄精多糖对脂肪组织分泌的生物活性物质致肝细胞HepG2分泌VRP的影响。方法:取脂肪组织培养液刺激肝细胞HepG2培养液,观察基础状态及黄精多糖对C-反应蛋白(CRP)分泌的影响。结果:内脏及皮下脂肪培养液均可刺激肝细胞HepG2产生CRP,且腹腔内脂肪培养液刺激HepG2合成CRP[(3.84±0.92)ng·mL-1]强于皮下脂肪[(2.45±0.75)ng·mL-1,P<0.01]。黄精多糖可抑制肝细胞HepG2分泌CRP,对皮下及腹腔脂肪培养液所产生的刺激作用分别抑制达45.8%和66.7%。结论:脂肪组织分泌的生物活性物质可刺激肝细胞HepG2分泌CRP,提示脂肪组织可通过CRP致动脉粥样硬化形成。黄精多糖可抑制CRP分泌,具有抗动脉粥样硬化作用。
OBJECTIVE: To observe the effect of Polygonatum sibiricum polysaccharide on secretion of VRP by hepatocyte HepG2 induced by bioactive substances secreted by adipose tissue. METHODS: Hepatocyte HepG2 medium was stimulated with adipose tissue culture fluid. The basal state and the effect of Huangjing polysaccharide on the secretion of C-reactive protein (CRP) were observed. RESULTS: Both visceral and subcutaneous fat culture media could stimulate hepatocyte HepG2 production of CRP, and intraperitoneal fat culture media stimulated HepG2 synthesis of CRP [(3.84±0.92) ng·mL-1] stronger than subcutaneous fat [(2.45±0.75) ng · mL-1, P<0.01]. Heteropolysaccharide can inhibit the secretion of CRP in hepatocyte HepG2, and the stimulatory effects on subcutaneous and abdominal fat culture fluids were inhibited by 45.8% and 66.7%, respectively. CONCLUSION: The bioactive substances secreted by adipose tissue can stimulate hepatocyte HepG2 secretion of CRP, suggesting that adipose tissue can cause atherosclerosis through CRP. Polygonatum polysaccharide can inhibit the secretion of CRP and has antiatherogenic effects.