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燃煤飘尘中含有许多具有细胞毒性和遗传毒性的金属。作者对同时存在于固体载体物(煤飘尘)上的金属经胎盘转运进行了研究。用静电聚尘器收集煤飘尘后,制备成颗粒直径≤5μm的煤飘尘样品,并分析其中金属。使用Wistar品系妊娠大鼠(220～240 g),从妊娠第14天起,给试验组妊娠大鼠100 mg/kg/日剂量的煤飘尘(以生理盐水配制成悬浮液),作气管内注入染毒,连续6天;同期内,给对照组妊娠大鼠气管内注入等容量生理盐水。于最后一次染毒后的次日,自妊娠大鼠颈静脉取血,制备血清;然后断头处死,摘取母
There are many cytotoxic and genotoxic metals in coal dust. The authors studied the transplacental transport of metals that coexist on solid supports (coal fly ash). After electrostatic dust collector was used to collect the dust of the coal, a sample of dust-free coal with a particle diameter of ≤5μm was prepared and the metal was analyzed. Wistar pregnant rats (220-240 g) were used. From the 14th day of pregnancy, 100 mg / kg / day of the pregnant rats in the experimental group were dusted with dust (prepared as a suspension with saline solution) for intratracheal instillation Exposure, continuous 6 days; over the same period, to the control group of pregnant rats injected with equal volume of saline tracheal. On the next day after the last exposure, blood was taken from the jugular vein of a pregnant rat to prepare serum; then, decapitation was performed and the mother was removed