论文部分内容阅读
目的 探讨组胺对肠粘膜屏障功能的影响。方法 采用体外单层 Ca Co- 2细胞和失血感染大鼠模型为对象 ,细菌易位数量为指标 ,观察组胺对肠粘膜屏障功能的影响。结果 1体外实验显示 ,经 1× 10 - 6 mol/L、1× 10 - 7mol/L和 1× 10 - 8mol/L 三种不同浓度的组胺处理过的 Ca Co- 2细胞液细菌培养结果分别为 8.2× 10 6 CFU/ml、4.8× 10 6 CFU/ml和14.3C× 10 6 FU /ml,其细菌易位数量明显低于对照组 (83.8× 10 6 CFU /ml) (P<0 .0 5 )。 2动物实验提示 ,组胺 (浓度为 1×10 - 6 mol/L)治疗组大鼠肝、淋巴结平均组织含菌量分别为 0 .88× 10 6 CFU/g1.6 9× 10 6 CFU/g。而对照组肝、淋巴结平均组织含菌量分别为 82 .6 2× 10 6 CFU/g和 48.46× 10 6 CFU/g。其组胺治疗组肝、淋巴结平均组织含菌量明显低于对照组 (P<0 .0 5 )。结论 小剂量组胺有抑制细菌进入上皮细胞 ,抑制肠道细菌易位的作用
Objective To investigate the effect of histamine on intestinal mucosal barrier function. Methods The monolayer Ca Co-2 cells in vitro and the rat model of hemorrhagic infection were used as targets, and the number of bacterial translocations was used as an index to observe the effect of histamine on the intestinal mucosal barrier function. Results 1 In vitro experiments showed that the results of bacterial culture of Ca Co-2 cells treated with 1 × 10 -6 mol / L, 1 × 10 -7 mol / L and 1 × 10 -8 mol / L histamine The bacterial translocations were 8.2 × 10 6 CFU / ml, 4.8 × 10 6 CFU / ml and 14.3 × 10 6 FU / ml, respectively. The number of bacterial translocations was significantly lower than that of the control group (83.8 × 10 6 CFU / ml) .0 5). 2 Animal experiments suggest that the average amount of bacteria in the liver and lymph node tissue of rats treated with histamine (1 × 10 -6 mol / L) is 0.88 × 10 6 CFU / g, 1.6 × 10 6 CFU / g. However, the mean amount of bacteria in the liver and lymph nodes of the control group was 82.62 × 10 6 CFU / g and 48.46 × 10 6 CFU / g, respectively. The histamine treatment group liver, lymph node average tissue content was significantly lower than the control group (P <0. 05). Conclusions Small doses of histamine can inhibit bacterial entry into epithelial cells and inhibit intestinal bacterial translocation