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为了查明土壤松紧度对Tullgren法分离小型节肢动物的影响,本研究设计了5种松紧度:在保持体积相同的情况下,土壤质量分别为原来的70%(容重1.03 g·cm-3)、80%(1.18 g·cm-3)、90%(1.33 g·cm-3)和100%(1.49 g·cm-3),并用原状土(1.47 g·cm-3)作对照。不同松紧度土样的分离结果为:每100 g土样中分离出的土壤动物个体数量呈波动性变化,大小顺序为70%>原状土>80%>90%>100%,其中松紧度为70%的土样分离到的个体数显著高于其他土样(P<0.05)。随着松紧度的降低,每100 g土样中分离到的甲螨亚目和弹尾目个体数量均显著增加(P<0.05或P<0.01),前气门亚目的数量无显著差异(P>0.05)。对土壤动物群落总体、甲螨亚目和弹尾目的分离速度随着松紧度的降低逐渐加快,而前气门亚目的分离速度无明显变化。研究结果表明,土壤松紧度对Tullgren法分离土壤动物的数量、群落结构及分离速度均有明显影响。
In order to find out the effect of soil tightness on the isolation of small arthropods by Tullgren method, we designed five kinds of tightness: the soil masses were 70% (1.03 g · cm-3) , 80% (1.18 g · cm-3), 90% (1.33 g · cm-3) and 100% (1.49 g · cm-3) respectively. The results of soil samples with different degrees of tightness were as follows: the number of soil animals isolated per 100 g of soil samples fluctuated in the order of 70%> undisturbed soil> 80%> 90%> 100% The number of individuals isolated from 70% soil samples was significantly higher than other soil samples (P <0.05). With the decrease of the tightness, the number of Entomoite and Cocosidia isolated per 100 g of soil samples increased significantly (P <0.05 or P <0.01), while there was no significant difference in the number of anterior valve sub-orders (P> 0.05 ). For the soil animal community as a whole, the speed of detachment of A. nipponensis and the tail was gradually accelerated with the decrease of the degree of tightness, while the separation speed of the anterior valve subfamily did not change significantly. The results show that the degree of soil elasticity has a significant effect on the quantity, community structure and separation speed of Tullgren method for separating soil animals.