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目的:探讨腹水离心石蜡切片及免疫组织化学在卵巢癌诊断中的价值。方法:对30例腹水标本离心收集细胞沉渣,经自动脱水机脱水后石蜡包埋,切片进行免疫组织化学检测CA125、CK7、Ki67表达。结果:腹水离心石蜡切片细胞形态清晰,着色鲜艳,免疫组织化学阳性定位准确。结论:腹水离心石蜡切片联合免疫组织化学检测CA125、CK7、Ki67对卵巢癌具有临床诊断价值。
Objective: To investigate the value of ascites centrifugal paraffin sections and immunohistochemistry in the diagnosis of ovarian cancer. Methods: Seventy ascites samples were centrifuged to collect cell sediment, dehydrated by an automatic dehydrator and embedded in paraffin. The expression of CA125, CK7 and Ki67 was detected by immunohistochemistry. Results: Ascitic fluid paraffin sections showed clear cell morphology, vivid staining and positive immunohistochemical localization. Conclusion: Centrifugal paraffin sections combined with immunohistochemical detection of CA125, CK7 and Ki67 have clinical value in the diagnosis of ovarian cancer.