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为探索可用于诊断的模拟表膜抗原。采用纯化的兔抗表膜抗原IgG作探针 ,免疫筛选噬菌体随机十二肽库 ,经 3轮生物淘洗后 ,随机挑选 30个噬菌体克隆 ,用ELISA检测其与筛选抗体的特异性结合 ,选择两个阳性克隆进行DNA序列测定 ,并用斑点ELISA比较检测正常人和日本血吸虫病患者血清各 10份。结果显示 ,随机挑选的 30个克隆中有 9个噬菌体克隆与筛选的抗体有特异性的结合反应。DNA测序结果显示 ,两个阳性噬菌体克隆 (携带的抗原表位 )所演绎的氨基酸序列与GenBank已知的氨基酸序列无同源性。斑点ELISA结果显示两个抗原表位可被血吸虫病患者血清呈特异性识别
In order to explore for the diagnosis of mimic epidermal antigens. Purified rabbit anti-membrane antigen IgG was used as a probe to immunoprecipitate a phage randomized 12-mer peptide library. After three rounds of bio-panning, 30 phage clones were randomly selected and their specific binding to the screened antibody was determined by ELISA Two positive clones were subjected to DNA sequencing and spot ELISA was used to detect 10 serums each of normal and Japanese schistosomiasis patients. The results showed that 9 out of 30 randomly selected clones had specific binding reaction with the selected antibodies. The results of DNA sequencing showed that the amino acid sequences deduced by the two positive phage clones (carrying antigen epitopes) have no homology with the known amino acid sequence of GenBank. Spot ELISA results showed that two epitopes were specifically recognized by the serum of schistosomiasis patients