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本研究应用ISSR分析了来自5个不同种源地的73份无患子种质的遗传多样性。ISSR共扩增出条带119条,其中多态性条带105条,多态性百分率为88.24%;表明不同地区的无患子种质间存在较高的多态性,遗传多样性较为丰富,各地区种质间的遗传差异较大;5个无患子种群间的遗传距离变化范围在0.032 4~0.081 0之间;UPGMA聚类分析表明,73份无患子种质可分为2大类,组A由安徽黄山、浙江台州、江西吉安和福建建宁组成;组B仅含江西龙南。分布于浙江台州与分布于江西吉安的无患子种质亲缘关系最近,分布于安徽黄山和江西龙南的无患子种质亲缘关系最远。ISSR方法能有效地阐明不同地区无患子种质的多样性,为更好地利用无患子优异种质资源进行育种提供参考。
In this study, ISSR was used to analyze the genetic diversity of 73 Sapindaceae germplasms from five different provenances. ISSR amplified a total of 119 bands, of which 105 polymorphic bands, the percentage of polymorphism was 88.24%; indicating that there is a high polymorphism in the germplasm in different regions of the germplasm, rich genetic diversity , And the genetic differences among germplasm in each region were quite large. The genetic distance among 5 Sapindus populations varied from 0.032 4 to 0.081 0. UPGMA cluster analysis showed that 73 Sapindaceae germplasms could be divided into 2 Group A consists of Huangshan in Anhui, Taizhou in Zhejiang, Ji’an in Jiangxi and Jianning in Fujian. Group B contains only Longnan in Jiangxi Province. Distributed in Taizhou, Zhejiang Province and Jiangxi Ji’Anapathy germplasm genetic relationship recently distributed in Huangshan, Anhui and Jiangxi Longnan Sapindaceae genetic relationship furthest. The ISSR method can effectively elucidate the diversity of Sapindaceae germplasm in different regions and provide a reference for better utilization of Sogota genetic resources.