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OBJECTIVE To investigate the effect of neuroprotective effect of lychee seed saponins(LSS) in BV-2.METHODS Aβ_(1-42) induced BV-2 cells were incubated with LSS for 12 h,the content of the inflammatory factors such as IL~(-1)β,TNF-α,COX-2 and i NOS in the supernatant of BV-2 cell were measured by ELISA.The detection of the m RNA levels and the protein expression of the inflammatory factors including IL~(-1)β,TNF-α,COX-2 and i NOS using real-time PCR and Western blotting,respectively.RESULTS The level of IL~(-1)β,COX-2 and i NOS significantly increased with the treatment of Aβ_(1-42),and 0.117 mg·L~(-1)-0.469 mg·L~(-1) LSS can inhibit these increased level.CONCLUSION LSS conferred neuroprotection via inhibiting the inflammatory factors expression.
OBJECTIVE To investigate the effect of neuroprotective effect of lychee seed saponins (LSS) in BV-2.METHODS Aβ_ (1-42) induced BV-2 cells were incubated with LSS for 12 h, the content of the inflammatory factors such as IL ~ (-1) β, TNF-α, COX-2 and i NOS in the supernatant of BV-2 cells were measured by ELISA. The detection of the m RNA levels and the protein expression of the inflammatory factors including IL ~ (-1 ) β, TNF-α, COX-2 and i NOS using real-time PCR and Western blotting, respectively.RESULTS The level of IL-1β, COX-2 and iNOS significantly increased with the treatment of Aβ_ ( 1-42), and 0.117 mg · L -1 -0.469 mg · L -1 LSS could inhibit these increased levels. CONCLUSION LSS conferred neuroprotection via inhibiting the inflammatory factors expression.