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目的:建立匹林咖敏片中马来酸氯苯那敏的RP-HPLC测定方法。方法:用Kromasil C18色谱柱(250 mm×4．6 mm,5μm),以乙腈-0．3％十二烷基硫酸钠溶液-磷酸(60:40:0．02)(用三乙胺调pH至3．5)为流动相,流速为1．0 ml·min-1,检测波长为264 nm。结果:马来酸氯苯那敏在20．21-60．63μg·ml-1范围内呈线性关系,r=0．999 9,平均回收率为99．7％,RSD= 0．4％(n=9)。结论:本法适用于匹林咖敏片中马来酸氯苯那敏的含量测定。
Objective: To establish a RP-HPLC method for determination of chlorphenamine maleate in METHODS: A Kromasil C18 column (250 mm × 4.6 mm, 5 μm) was used with acetonitrile-0.3% sodium dodecyl sulfate-phosphoric acid (60:40:.02) pH to 3.5) as the mobile phase at a flow rate of 1.0 ml · min-1 with a detection wavelength of 264 nm. Results: Chlorphenamine maleate showed a linear relationship in the range of 20.21-60.63μg · ml-1, r = 0.999 9, with an average recovery of 99.7% and RSD = 0.4% ( n = 9). Conclusion: This method is suitable for the determination of chlorpheniramine maleate in.