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目的:简单研究复方黄柏液及其组方提取成分、水部位、醇部位体外对呼吸道合胞病毒、单纯疱疹病毒1型、肠道病毒71型3种病毒的抑制作用。方法:采用致细胞病变效应法(CPE法),进行体外抗病毒试验,逐日观察细胞病变,计算得出抑毒指数。结果:复方黄柏液对3种病毒的抑毒指数是32、16、128。结论:复方黄柏液对呼吸道合胞病毒、单纯疱疹病毒1型以及肠道病毒71型均具有明显的抑制作用,并且抗病毒效果呈现:EV71>RSV>HSV-1,即复方黄柏液对EV71的作用效果最好。
OBJECTIVE: To simply study the inhibitory effects of extracts of Cortex Phellodendri and its constituents, water fractions and alcohol fractions on three viruses, respiratory syncytial virus, herpes simplex virus type 1 and enterovirus 71 in vitro. Methods: The cytopathic effect (CPE) method was used to test the antiviral activity in vitro. The cytopathic effect was observed daily and the anti-toxin index was calculated. Results: The anti-virus index of compound Huangbai liquid to three kinds of viruses was 32,16,128. CONCLUSION: Phellodendron chinense has obvious inhibitory effect on respiratory syncytial virus, herpes simplex virus type 1 and enterovirus 71, and the anti-virus effect is as follows: EV71> RSV> HSV-1, The best effect.