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目的:研究古典型鼠疫菌与假结核菌ATCC29833基因组间的差异,为进一步认识鼠疫菌的起源与进化奠定基础。方法:通过抑制消减杂交技术比较两种细菌基因组间的差异。结果:与已测序的 3株鼠疫菌基因组进行同源性比较,发现了 259个假结核菌基因组中存在的特异序列,与基因库进行同源性比较发现了 10个假结核菌ATCC29833基因组中存在的新序列。结论:抑制消减杂交技术是一个简单而有效的比较近源微生物基因组之间差异的方法,鼠疫菌在进化过程中失去了大量的基因,假结核菌基因组之间也存在着一定的差别。
Objective: To study the difference between the genomes of classical Yersinia pestis and pseudotuberculosis ATCC29833, so as to lay a foundation for further understanding the origin and evolution of Y. pestis. Methods: The differences between the two bacterial genomes were compared by suppression subtractive hybridization. Results: Compared with the sequenced genomes of three strains of Y. pestis, homology comparison showed that there were 259 specific sequences in the genome of Pseudomonas solani. The homology comparison with the gene pool showed that there were 10 genomes in the genome of ATCC29833 The new sequence. Conclusion: Suppression subtractive hybridization (SSH) is a simple and effective method to compare the genomes of near-living microorganisms. Yersinia pestis lost a large number of genes during the evolutionary process. There are also some differences between the genomes of pseudotuberculosis.