野生毛葡萄芽器官离体培养试验结果，外植体——茎段芽在含有植物生长调节剂ＢＡ２ｍｇ～４ｍｇ／Ｌ、ＩＡＡ１．０ｍｇ／Ｌ的ＭＳ培养基上，被直接诱导出不定芽，芽继代培养分化率高，但芽茎粗且短，呈密集型，ＢＡ和ＩＡＡ减半的Ｍｓ培养基，促进形成正常健壮芽。在含有ＩＢＡ０．５ｍｇ／Ｌ的ＭＳ培养基上，无根苗生长１０天后开始长出根点，１５天根系长至０．５～１ｃｍ。移栽于经０．１％高锰酸钾溶液消毒的基质里，成活率９０％，生长健壮，培育成完整的小植株。 In vitro culture of wild Wort buds showed that explants-shoot buds were directly induced on MS medium containing plant growth regulators BA2mg ~ 4mg / L, IAA1.0mg / L, and adventitious buds and buds Subculturing Ms medium with high differentiation rate, but with thick and short buds, dense, BA and IAA halves, promoted the formation of normal and robust shoots. On MS medium containing 0.5 mg / L IBA, root growth began to develop after 10 days of root growth, and roots grew to 0.5 to 1 cm on the 15th day. Transplanted in 0.1% potassium permanganate solution disinfection of the matrix, the survival rate of 90%, robust growth, cultivate into a complete plantlets.