目的研究半边铁角蕨(Asplenium unilaterale Lam.)全草化学成分。方法全草采用甲醇提取,利用硅胶、Sephadex LH-20、半制备HPLC等色谱技术方法进行分离纯化,根据波谱数据对化合物进行结构鉴定。结果分离并鉴定了15个化合物,分别为(Z)-4-(2,6,6-三甲基亚环己-2-烯基)丁-2-醇(1)、淫羊藿次苷B_2(2)、柑橘苷A(3)、6R,9R-3-酮-α-紫罗兰醇-9-O-β-D-吡喃葡萄糖苷(4)、(6S,9R)-6-羟基-3-酮-α-紫罗兰醇-9-O-β-D-葡萄糖苷(5)、6-甲基-香豆素(6)、6,7-二羟基-香豆素(7)、gibepyrone D(8)、羽扇豆烷-3-酮(9)、佛来心苷(10)、22,23-二甲基胆甾-5-烯-3-醇(11)、正三十二醇(12)、5-己烷呋喃-2(5H)-酮(13)、托可醌(14)和2,4(1H,3H)-嘧啶二酮(15)。结论化合物1~15均为首次从该属植物中分离得到。 Objective To study the chemical constituents of Asplenium unilaterale Lam. Methods The whole plant was extracted with methanol and separated by silica gel, Sephadex LH-20, semi-preparative HPLC and other chromatographic techniques. The structures of the compounds were identified by spectral data. Results Fifteen compounds were isolated and identified as (Z) -4- (2,6,6-trimethylcyclohex-2-enyl) butan-2-ol (2), citrus glycosides A (3), 6R, 9R-3-keto-α-ionol-9-O-β-D- glucopyranoside (4), (6S, 9R) (5), 6-methyl-coumarin (6), 6,7-dihydroxy-coumarin (7), 6-methyl- gibepyrone D (8), lupine-3-one (9), beraprost (10), 22,23-dimethylcholest-5-en- Alcohol (12), 5-hexane furan-2 (5H) -one (13), tropicolone (14) and 2,4 (1H, 3H) -pyrimidinedione (15). Conclusion Compounds 1 ~ 15 were isolated from this genus for the first time.