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为了解中国广州、吉林、成都三个地区汉族和日本人群体D19540O基因座基因频率分布，并获得中国三个汉族群体和日本群体D19M00基因座的群体遗传数据，比较它们之间的遗传学差异，探究在法医学应用中的意义。应用PCR扩增技术，聚丙烯酸胺凝胶垂直板电泳对D19S400基因座分型。在四个群体469个个体中共检出11个等位基因，45种基因型，基因型频率分布均符合Hardy-Weinberg平衡。各群体的观察杂合度为0.75～0.84，非父排除概率为0.6057～0.6582，个人识别机率为0．9301～0．9480。四个群体之间基因频率分布无显著性差异（P＞0．05）。结果表明，D19S400基因座在群体遗传学研究和法医学个人识别中有较高应用价值。
To understand the frequency distribution of D19540O gene loci in Han and Japanese populations in three areas of Guangzhou, Jilin and Chengdu, China, population genetic data of D19M00 locus in three Han Chinese and Japanese populations in China were obtained. The genetic differences among them were compared. Exploring the significance of forensic application. The D19S400 locus was typed by polyacrylamide gel electrophoresis with polyacrylamide gel electrophoresis. Eleven alleles were detected in 469 individuals from four populations. Forty-five genotypes were detected, and the genotype frequency distribution was in accordance with Hardy-Weinberg equilibrium. The observed heterozygosities of all groups ranged from 0.75 to 0.84, the probability of non-parent exclusion was 0.6057 to 0.6582, and the probability of personal identification was 0.9301 to 0.9480. There was no significant difference in gene frequency among the four groups (P> 0.05). The results showed that the D19S400 locus has high value in population genetics and forensic personal identification.