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本文报告了~(14)N和~4He离子束所致离体早代金黄地鼠胚胎细胞(GHE)的杀伤作用和转化作用.并与~(60)Coγ射线进行了比较.以胰酶消化的第13～14天GHE为靶细胞,在含有10%胎牛血清的Eagle’s MEM培养基内37℃、5%CO_2条件下培养.重离子源由回旋加速器产生.样品处的离子能量和LET值分别是:3.1MeV/amu和530 keV/μm(~(14)N)、4.2 MeV/amu和36keV/μm(~4He)、1.7 MeV/amu和77kcV/μm(加铝吸收板的~4He).剂量的变化范围是:0.7～1.0Gy/min(~(14)N)、1.0～2.6Gy/min(~4He).~(60)Coγ射线的
This paper reports the killing and transformation of isolated early golden hamster embryonic cells (GHE) induced by ~(14)N and ~4He ion beams, and compared them with ~(60)Co gamma rays. On the 13th to the 14th day, GHE cells were used as target cells and cultured in Eagle’s MEM medium containing 10% fetal bovine serum at 37°C and 5% CO 2 . Heavy ion sources were generated by a cyclotron. The ion energy and LET value at the sample were measured. They are: 3.1 MeV/amu and 530 keV/μm (~14N), 4.2 MeV/amu, and 36 keV/μm (~4He), 1.7 MeV/amu, and 77 kcV/μm (~4He with aluminum absorption plate) The range of dosage is: 0.7～1.0Gy/min(~14N), 1.0～2.6Gy/min(~4He).~(60)Coγ-ray