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为了调查HIV 1感染相关的等位基因CCR5△32、CCR2b 64I、SDF1 3′A在我国云南省德宏州傣族景颇族人群中的频率和多态性分布,此课题以101例傣族和113例景颇族人群为研究对象,应用PCR、PCR RFLP(聚合酶链反应 限制性片段长度多态性)分析方法进行检测,计算突变基因频率;并对其群体分布、性别分布进行统计学分析。结果表明,中国傣族景颇族人群中未发现CCR5△32等位基因突变;傣族CCR2b 64I、SDF1 3′A基因突变频率分别为0.2130和0.2030,景颇族CCR2b 64I和SDF1 3′A基因突变频率分别为0.1637和0.1770;与中国汉族人群相比较,傣族和景颇族中SDF1 3′A突变频率较低(P值分别为0.0322和0.0021);两个民族的CCR2b 64I和SDF1 3′A等位基因群体分布符合Hardy Weinberg平衡,在性别之间分布无显著差异。中国傣族景颇族人群的CCR2b 64I等位基因的突变频率与汉族人相似,SDF1 3′A等位基因的突变频率比汉族人低,此两种突变基因在艾滋病发病过程中的影响值得进一步研究。由于未发现CCR5△32基因突变,中国傣族景颇族人群对HIV 1感染可能有较大的遗传易感性。
In order to investigate the frequency and polymorphism distribution of CCR5 △ 32, CCR2b 64I and SDF1 3’A alleles associated with HIV 1 infection in the Dai and Jingpo ethnic groups in Dehong Prefecture, Yunnan Province, this study investigated 101 cases of Dai and 113 cases The population of Jingpo people were detected by PCR and PCR RFLP (restriction fragment length polymorphism). The frequencies of the mutated genes were calculated. The population distribution and gender distribution were analyzed statistically. The frequency of CCR2b 64I and SDF1 3’A gene mutations in Dai nationality was 0.2130 and 0.2030, respectively. The frequencies of CCR2b 64I and SDF1 3’A gene mutations in Jingpo ethnic group were 0.1637 and 0.1770, respectively. Compared with Chinese Han population, the frequency of SDF1 3’A mutation was lower in Dai and Jingpo (P values were 0.0322 and 0.0021 respectively). The distribution of CCR2b 64I and SDF1 3’A alleles in two ethnic groups In line with the Hardy Weinberg equilibrium, there was no significant difference between the sexes. The frequencies of the CCR2b 64I allele in the Chinese people of the Dai and Jingpo ethnic groups in China are similar to those of the Han nationality. The mutation frequency of the SDF1 3’A allele is lower than that of the Han nationality. The effects of the two mutations in the pathogenesis of AIDS deserve further study. Since no CCR5 △ 32 gene mutation was found, the population of Jingpo people of Dai nationality in China may have greater genetic susceptibility to HIV-1 infection.