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Bacterial decolorization of anthraquinone dye intermediates is a slow process under aerobic conditions.To speed up the process,in the present study,effects of various nutrients on 1-amino-4-bromoanthraquinone-2-sulfonic acid (ABAS) decolorization by Sphingomonas xenophaga QYY were investigated.The results showed that peptone,yeast extract and casamino acid amendments promoted ABAS bio-decolorization.In particular,the addition of peptone and casamino acids could improve the decolorization activity of strain QYY.Further experiments showed that L-proline had a more significant accelerating effect on ABAS decolorization compared with other amino acids.L-Proline not only supported cell growth,but also significantly increased the decolorization activity of strain QYY.Membrane proteins of strain QYY exhibited ABAS decolorization activities in the presence of L-proline or reduced nicotinamide adenine dinucleotide,while this behavior was not observed in the presence of other amino acids.Moreover,the positive correlation between L-proline concentration and the decolorization activity of membrane proteins was observed,indicating that L-proline plays an important role in ABAS decolorization.The above findings provide us not only a novel insight into bacterial ABAS decolorization,but also an L-proline-supplemented bioaugmentation strategy for enhancing ABAS bio-decolorization.