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AIM:To determine if anisodamine is able to augmentmucosal perfusion during gut I/R ischemia-reperfusion.METHODS:A jejunal sac was formed in Sprague Dawley rat.A Laser Doppler probe and a tonometer were inserted intothe sac which was filled with saline.The superiormesenteric artery was occluded(SMAO)for 60 minutesfollowed by 90 minutes of reperfusion.At the end of 60minutes of SMAO,either 0.2 mg/kg of anisodmine ordobutamine was injected into the jejunal sac.Laser Dopplermucosal blood flow and regional PCO_2(PrCO_2)measurements were made.RESULTS:Mucosal blood flow was significantly increased at30,60 and 90 minutes of reperfusion(R_(30),R_(60),R_(90))whenintraluminal anisodamine or dobutamine was presentcompared to intraluminal saline only(44±3.3% or 48±4.1% vs 37±2.6% at R_(30),57±5.0% or 56±4.7% vs 45±2.7% at R_(60),64±3.3% or 56±4.2% vs 48±3.4% at R_(90),respectively P<0.05).Blood flow changes were alsoreflected by lowering of jejunal PrCO_2 measurements afterintraluminal anisodamine or dobutamine compared with thatof the saline controls(41±3.1 mmHg or 44±3.0 mmHg vs49±3.7 mmHg at R_(30),38±3.7 mmHg or 40±2.1 mmHg vs 47±3.8 mmHg at R_(60),34±2.1 mmHg or 39±3.0 mmHg vs 46±3.4 mmHg at R_(90),respectively,P<0.05).Most interestingfinding was that there were significantly higher mucosalblood flow and lower jejunal PrCO_2 in anisodamine groupthan those in dobutamine group at 90 minutes of reperfusion(64±3.3% vs 56±4.2% for blood flow or 34±2.1 mmHgvs 39±3.0 mmHg for PrCO_2,respectively,P<0.05),suggesting that anisodamine had a more lasting effect onmucosal perfusion than dobutamine.CONCLUSION:Intraluminal anisodamine and dobutaminecan augment mucosal blood flow during gut I/R and alleviatemucosal acidosis.The results provided benificial effects onthe treatment of splanchnic hypoperfusion followingtraumatic or burn shock.
AIM: To determine if anisodamine is able to augment mucosal perfusion during gut I / R ischemia-reperfusion. METHODS: A jejunal sac was formed in Sprague Dawley rat. A Laser Doppler probe and a tonometer were inserted intothe sac which was filled with saline. The end of 60 minutes of SMAO, either 0.2 mg / kg of anisodmine ordobutamine was injected into the jejunal sac. Laser Doppler mucosal blood flow and regional PCO 2 (PrCO 2) measurements were made. RESULTS: Mucosal blood flow was significantly increased at 30, 60 and 90 minutes of reperfusion (R_ (30), R_ (60), R_ (90)) whenintraluminal anisodamine or dobutamine was presentcompared to intraluminal saline only (44 ± 3.3% or 48 ± 4.1% vs 37 ± 2.6% at R_ (30), 57 ± 5.0% or 56 ± 4.7% vs 45 ± 2.7% at R_ (60), 64 ± 3.3% or 56 ± 4.2% vs 48 ± 3.4% at R_ (90), respectively P <0.05) .Blood flow changes were alsoreflected by lowering of jejunal PrCO_2 measurements after intraluminal ani sodamine or dobutamine compared with that of the saline controls (41 ± 3.1 mmHg or 44 ± 3.0 mmHg vs 49 ± 3.7 mmHg at R_ (30), 38 ± 3.7 mmHg or 40 ± 2.1 mmHg vs 47 ± 3.8 mmHg at R_ (60), 34 ± 2.1 mmHg or 39 ± 3.0 mmHg vs. 46 ± 3.4 mmHg at R_ (90), respectively, P <0.05). Studliedfinding was that there were significantly higher mucosalblood flow and lower jejunal PrCO_2 in anisodamine groupthan those in dobutamine group at 90 minutes suggesting that anisodamine had a more lasting effect on mucosal perfusion than dobutamine. CONCLUSION: Intraluminal (64 ± 3.3% vs 56 ± 4.2% for blood flow or 34 ± 2.1 mmHg vs. 39 ± 3.0 mmHg for PrCO_2, respectively, P <0.05) anisodamine and dobutaminecan augment mucosal blood flow during gut I / R and alleviate mucosal acidosis. The results provided benificial effects on the treatment of splanchnic hypoperfusion following traumatic or burn shock.