论文部分内容阅读
材料和方法:干燥穿心莲茎叶用80％水醇(80％乙醇∶20％双蒸水)提取3次,提取液冻干后4℃保存备用。选用雄性Swiss albino小鼠(6～8周龄),分为4组,即空白对照组、穿心莲提取物50mg/kg剂量组、100mg/kg剂量组、阳性对照药丁羟茴醚(BHA)组,连续给药14d后,禁食1夜,处死动物,取出肝脏,制备匀浆,离心。上清液用于测定细胞溶质GST、
Materials and Methods: Dried and dried shoots of Andrographis paniculata were extracted 3 times with 80% hydro alcohol (80% ethanol: 20% double distilled water). The extract was freeze-dried and stored at 4°C until use. Male Swiss albino mice (6-8 weeks old) were selected and divided into 4 groups: blank control group, Andrographis paniculata extract 50 mg/kg dose group, 100 mg/kg dose group, and positive control group butylated hydroxyanisole (BHA) group. After 14 days of continuous administration, the animals were sacrificed overnight and the animals were sacrificed. The livers were removed to prepare homogenates and centrifuged. The supernatant was used to determine the cytosolic GST,