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研究了吐温－８０存在下，Ｓｅ（Ⅳ）与碘化物和罗丹明Ｂ形成三元离子缔合物分光光度测定硒的方法，该方法应用于尿液、血清样中微量硒测定，结果满意。
The method for the spectrophotometric determination of selenium with Se (Ⅳ) and iodide and rhodamine B in the presence of Tween-80 was developed. The method was applied to the determination of trace selenium in urine and serum samples with satisfactory results .