论文部分内容阅读
以NaCl为筛选剂,对5个大豆品种的子叶节诱导、丛生芽生根及大豆苗期的生长情况进行研究。结果表明:不同基因型大豆对NaCl的敏感性不同,同一基因型的不同部位对NaCl的敏感性也存在差异。最终确定了NaCl对子叶节分化、丛生芽生根以及苗期生长适宜的筛选浓度。大豆子叶节分化NaCl临界筛选浓度:吉农27和吉农17为225 mmol.L-1;吉林30和DE2259为200 mmol.L-1;吉农28为175 mmol.L-1。丛生芽生根NaCl临界筛选浓度:吉农27和吉农17为100 mmol.L-1;吉林30、DE2259和吉农28为75 mmol.L-1。大豆苗期NaCl临界筛选浓度:吉农27、吉农17、吉林30和DE2259均为250 mmol.L-1;吉农28为200 mmol.L-1。
NaCl as the screening agent, the five soybean varieties induced by cotyledonary node, shoots buds and soybean seedling growth were studied. The results showed that different genotypes of soybean had different sensitivity to NaCl, and different parts of the same genotype had different sensitivity to NaCl. Finally, the appropriate screening concentration of NaCl for cotyledonary node differentiation, rooting of clustered buds and seedling growth was determined. The critical screening concentration of NaCl in soybean cotyledons was 225 mmol.L-1 in JiNan 27 and Jinong 17, 200 mmol·L-1 in Jilin 30 and DE2259, and 175 mmol·L-1 in JiNong 28. The critical screening NaCl concentration for rooting of shoots was 100 mmol.L-1 for JiNan 27 and Jinong 17, and 75 mmol.L-1 for Jilin 30, DE2259 and JiNin 28. The critical screening concentration of NaCl in soybean seedlings was 250 mmol.L-1 for JiNan 27, JiNan 17, Jilin 30 and DE2259, and 200 mmol.L-1 for JiNong 28.