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本研究利用添加荔枝枝屑的筛选培养基,通过常压室温等离子体(ARTP)诱变技术对柱状田头菇(茶树菇)菌丝体进行诱变处理,共获得67株再生菌株,初筛得到12株再生菌株与出发菌株有较明显的拮抗反应,复筛得到7株再生菌株的长速、长势优于出发菌株,并通过酯酶同工酶电泳技术对这7株再生菌株进行菌种的真实性鉴定。结果确定了一株适宜分解荔枝枝屑的优良再生菌株AL20,其与出发菌株的酯酶同工酶谱联合系数为0.60,是产生了遗传距离的新菌株,且其菌丝长速达3.13mm/d,生物学效率达到73.15%,分别较出发菌株提高0.21mm/d、9.03%,因此初步确定AL20是能够高效利用荔枝枝屑生产的新菌株。
In this study, the mycelium of Agrocybe aegerita was mutagenized by atmospheric pressure room-temperature plasma (ARTP) mutagenesis using screening media with the addition of lychee-tree chips. A total of 67 strains of regenerated strains were obtained, Twelve regenerated strains were found to have obvious antagonistic reaction with the starting strain. The seven strains of regenerated strains grew faster and grew better than the original strains by the screen-rescreening method. The strains of seven regenerated strains were tested by esterase isoenzyme electrophoresis The authenticity of the identification. The result showed that AL20, an excellent regeneration strain suitable for lychee detritus, was identified. The coefficient of combination with esterase isozyme of the original strain was 0.60, which was a new strain with genetic distance, and its mycelial length reached 3.13mm / d. The biological efficiency reached 73.15%, which was 0.21mm / d and 9.03% higher than that of the original strain. Therefore, it was initially determined that AL20 was a new strain capable of utilizing lychee and detritus efficiently.