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目的 以血清流行病学方法调查新疆出血热 (XHF)病人、易感人群和主要宿主动物中疾病的感染情况。方法 分别收集 2 0 0 1年 4～ 6月新疆巴楚县临床诊断为XHF的病人血清、易感人群血清和主要宿主动物的血清 ,用研制的诊断试剂以酶联免疫吸附试验 (ELISA)检测XHF特异性IgG和IgM抗体 ;用抗原捕获ELISA检测XHF病毒抗原。 结果 病人血清IgG抗体阳性率为39 .6 2 % (2 1/ 5 3) ,IgM抗体阳性率为2 0 .75 % (11/ 5 3) ,抗原捕获ELISA有 1份血清为XHF抗原阳性 ;易感人群血清IgG抗体阳性率为 2 1.0 5 % (4/ 19) ,IgM抗体检测和抗原捕获ELISA全部为阴性 ;羊血清IgG抗体阳性率为 70 % (5 6 / 80 )。 结论 血清流行病学研究证实该次疫情确系XHF ,流行地区人畜均有较高水平的隐性感染。
Objective To investigate the prevalence of disease in Xinjiang hemorrhagic fever (XHF) patients, susceptible populations and major host animals using serological epidemiological methods. Methods The sera from serum and susceptible population of patients diagnosed as XHF in Bachu County, Xinjiang from April to June in 2001 were collected respectively and the serum of the main host animals were collected. The developed diagnostic reagents were detected by enzyme - linked immunosorbent assay (ELISA) XHF-specific IgG and IgM antibodies; Antigen capture ELISA to detect XHF virus antigens. Results The positive rate of serum IgG antibody was 39.62% (2 1/5 3), the positive rate of IgM antibody was 20.575% (11/53), and 1 serum of antigen capture ELISA was positive for XHF antigen. The positive rate of serum IgG antibody in susceptible population was 2 1.05% (4/19), all of IgM antibody detection and antigen capture ELISA were negative. The positive rate of IgG antibody in goat serum was 70% (56/80). Conclusions Seroepidemiological studies confirmed that the outbreak was confirmed to be XHF, with a high level of latent infection in both endemic and endemic areas.