论文部分内容阅读
目的:探讨子宫内膜癌中脆性组氨酸三联体(FHIT)基因的表达及其与临床病理指标的可能关系。方法:采用免疫组化S-P法检测36例子宫内膜癌、17例子宫内膜上皮内瘤变、14例正常子宫内膜组织标本中FHIT的表达。结果:子宫内膜癌、子宫内膜上皮内瘤变及正常子宫内膜组织中FHIT的阳性表达率分别为50%(18/36)、88.26%(15/17)和92.86%(13/14);正常子宫内膜与子宫内膜瘤变组织中FHIT阳性表达率比较,差异无显著性(P>0.05);正常子宫内膜、子宫内膜瘤变分别与子宫内膜癌组织FHIT阳性表达率比较,差异均有显著性(P<0.01)。FHIT阳性表达在子宫内膜癌组织学分级的分布为Ⅰ级57.89%,Ⅱ级50%,Ⅲ级28.57%,各分级组间比较差异无显著性(P>0.05)。FHIT阳性表达在子宫内膜癌手术病理分期的分布为Ⅰ期64%、Ⅱ期20%、Ⅲ~Ⅳ期16.67%,三组间比较差异无显著性(P>0.05)。子宫内膜癌有淋巴结转移组与无淋巴结转移组FHIT的阳性表达率分别为16.67%(1/6)和66.67%(14/21),差异无显著性(P>0.05)。子宫内膜癌中浅肌层侵润与深肌层侵润FHIT阳性表达率分别为65%和31.25%,两者比较差异无显著性(P>0.05)。结论:子宫内膜组织中FHIT基因表达降低可能与子宫内膜癌的发生有关,但与手术病理分期、肌层侵润深度及淋巴结转移无关,该基因在子宫内膜癌组织中表达降低进一步揭示了子宫内膜癌的发病机制。
Objective: To investigate the expression of FHIT gene in endometrial carcinoma and its possible relationship with clinicopathological parameters. Methods: The expression of FHIT in 36 cases of endometrial carcinoma, 17 cases of endometrial intraepithelial neoplasia and 14 cases of normal endometrial tissue were detected by immunohistochemical S-P method. Results: The positive rates of FHIT in endometrial carcinoma, endometrial intraepithelial neoplasia and normal endometrium were 50% (18/36), 88.26% (15/17) and 92.86% (13/14) respectively ). There was no significant difference in FHIT positive expression rate between normal endometrium and endometrial neoplasia (P> 0.05). Normal endometrium and endometrial neoplasia were positively correlated with FHIT expression in endometrial carcinoma Rates were significantly different (P <0.01). FHIT positive expression in endometrial carcinoma histological grading distribution of grade Ⅰ 57.89%, Ⅱ grade 50%, Ⅲ grade 28.57%, there was no significant difference between the grading groups (P> 0.05). The distribution of FHIT positive in endometrial carcinoma was 64% in stage Ⅰ, 20% in stage Ⅱ, and 16.67% in stage Ⅲ ~ Ⅳ. There was no significant difference between the three groups (P> 0.05). The positive rates of FHIT in patients with endometrial carcinoma with lymph node metastasis and without lymph node metastasis were 16.67% (1/6) and 66.67% (14/21) respectively, with no significant difference (P> 0.05). The positive rates of FHIT in superficial myometrial invasion and deep myometrial invasion in endometrial carcinoma were 65% and 31.25%, respectively. There was no significant difference between the two groups (P> 0.05). Conclusion: The decrease of FHIT gene expression in endometrial tissue may be related to the occurrence of endometrial carcinoma. However, the expression of FHIT gene in endometrial carcinoma is not related to the pathological stage, muscular invasion depth and lymph node metastasis. The pathogenesis of endometrial cancer.