论文部分内容阅读
目的探讨宫颈脱落细胞中人类染色体端粒酶mRNA基因(hTERC)扩增与宫颈病变程度及不同亚型人乳头瘤病毒(HPV)感染的关系。方法宫颈上皮内瘤样变(CIN)患者34例,其中CINⅠ8例,CINⅡ9例,CINⅢ(包括宫颈原位鳞癌)17例;浸润型宫颈鳞癌(ISCC)36例;慢性宫颈炎患者20例。对上述患者液基薄层细胞学检测(TCT)剩余样本应用荧光原位杂交技术(FISH)检测hTERC基因,快速导流杂交基因芯片技术检测HPV感染亚型,分析hTERC扩增与宫颈病变程度、HPV感染的关系。结果慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ、ISCC中hTERC扩增率分别为0.00%(0/20)、50.00%(4/8)、77.78%(7/9)、82.35%(14/17)和97.22%(35/36),随着宫颈病变程度的增加,hTERC扩增率增高,组间两两比较差异均有统计学意义(P<0.05);慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ、ISCC中HPV感染率分别为10.00%(2/20)、37.50%(3/8)、66.67%(6/9)、88.24%(15/17)、91.67%(33/36);HPV16型阳性、其他高危型阳性、阴性/低危型阳性组中hTERC扩增率分别为90.38%(47/52)、66.67%(4/6)、28.13%(9/32),组间比较差异具有统计学意义(P<0.01)。结论 hTERC扩增与宫颈病变的进展和HPV感染密切相关;HPV16型感染可能是导致高级别宫颈病变的主要原因,HPV58、33、52型在CIN及ISCC病变中也有一定优势作用。
Objective To investigate the relationship between human telomerase reverse transcriptase gene (hTERC) amplification and the degree of cervical lesions and HPV subtypes in cervical exfoliated cells. Methods Twenty-four patients with cervical intraepithelial neoplasia (CIN), including 8 cases of CINⅠ, 9 cases of CINⅡ, 17 cases of CINⅢ (including squamous carcinoma of the cervix), 36 cases of invasive squamous cell carcinoma of the cervix (ISCC), 20 cases of chronic cervicitis . The hTERC gene was detected by fluorescence in situ hybridization (FISH) and the HPV subtype was detected by rapid flow-through hybridization gene chip in remaining samples of liquid-based TLCT. The hTERC amplification and cervical lesions were analyzed. The relationship between HPV infection. Results The hTERC amplification rates in chronic cervicitis, CINⅠ, CINⅡ, CINⅢ and ISCC were 0.00% (0/20), 50.00% (4/8), 77.78% (7/9) and 82.35% And 97.22% (35/36), respectively. The rate of hTERC expansion was increased with the increase of cervical lesions, and there was significant difference between groups (P <0.05). Chronic cervicitis, CINⅠ, CINⅡ, CINⅢ, HPV infection rates in ISCC were 10.00% (2/20), 37.50% (3/8), 66.67% (6/9), 88.24% (15/17) and 91.67% (33/36) . The hTERC amplification rates in other high-risk positive and negative / low-risk positive groups were 90.38% (47/52), 66.67% (4/6) and 28.13% (9/32), respectively. The difference between the two groups was statistically significant Significance (P <0.01). Conclusion The hTERC amplification is closely related to the progression of cervical lesions and HPV infection. HPV16 infection may be the main cause of high-grade cervical lesions. HPV58, 33 and 52 also have some predominant effects on CIN and ISCC.