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目的建立同时测定美容美发工作场所空气中12种芳香胺染料成分的溶剂解吸—液相色谱方法。方法工作场所空气中的芳香胺采用硅胶管采集,用10%乙腈的水溶液(pH=3.5)解吸后进样,通过液相色谱—二级阵列管检测器(HPLC—DAD)分离测定。结果该法中12种芳香胺有较好的分离度,线性相关系数在0.9990~1.0000之间,线性范围在0.5 mg/L~200 mg/L至2 mg/L~200 mg/L之间,平均相对标准偏差(RSD)为1.6%~8.3%,最低检出质量浓度为0.02 mg/m3~0.12 mg/m3(以采集12 L工作场所空气计),平均解吸效率为91.7%~100%,采样效率为92.4%~100%,100 mg硅胶管对12种芳香胺的穿透容量为643.7μg~1143μg,样品在4℃避光冷藏条件下可保存3 d。结论该法操作简便,准确度、精密度及解析效率均满足要求,适用美容美发工作场所空气中多种芳香胺的测定。
OBJECTIVE To establish a solvent desorption-liquid chromatographic method for the simultaneous determination of 12 aromatic amine dyes in the air of the beauty salon. Methods Aromatic amines in the air of the workplace were collected with a silica gel tube and desorbed with 10% acetonitrile in water (pH = 3.5). The samples were separated and determined by HPLC-DAD. Results The results showed that 12 kinds of aromatic amines had good resolution. The linear correlation coefficients ranged from 0.9990 to 1.0000 with a linear range of 0.5 mg / L ~ 200 mg / L to 2 mg / L ~ 200 mg / L, The average relative standard deviations (RSDs) ranged from 1.6% to 8.3% with the minimum detectable concentrations from 0.02 mg / m3 to 0.12 mg / m3 for 12 L workplace air. The average desorption efficiency was 91.7% -100% The sampling efficiency was 92.4% ~ 100%. The permeation capacity of 100 mg silica gel tubes for the 12 aromatic amines was 643.7μg ~ 1143μg. The samples were stored at 4 ℃ for 3 days in the light-dark condition. Conclusion The method is simple, accurate, precise and analytical efficiency to meet the requirements of the beauty salon for a variety of aromatic amines in the air.