[Objective] To study the electrochemical behavior of antitumor herbal drug apigenin and the interaction of apigenin with DNA at glassy carbon electrode. [Method] Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used in experiments. [Result] In CV, only one irreversible anodic peak with Ep = 0.580V was appeared in 0.1 mol/L B-R buffer solution (50% ethanol, pH 9.0). The peak currents of apigenin were linearly related to concentrations in a range from 5.0×10-6 to 9.0×10-5 mol/L. And the detection limit was 1.5×10-6 mol/L. A new electroanalytical method for this herbal drug was established according to this anodic peak. Using the established method without preseparation, apigenin in herbal drug was determined with satisfactory results. [Conclusion] This method is a new one to detect apigenin. [Objective] To study the electrochemical behavior of antitumor herbal drug apigenin and the interaction of apigenin with DNA at glassy carbon electrode. [Method] Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used in experiments. , only one irreversible anodic peak with Ep = 0.580 V was appeared in 0.1 mol / L BR buffer solution (50% ethanol, pH 9.0). The peak currents of apigenin were linearly related to concentrations in a range from 5.0 × 10-6 to 9.0 × 10 -5 mol / L And the detection limit was 1.5 × 10 -6 mol / L. A new electroanalytical method for this herbal drug was established according to this anodic peak. Using the established method without preseparation, apigenin in herbal drug was determined with satisfactory results. [Conclusion] This method is a new one to detect apigenin.