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多糖是黄芪的重要成分,但多糖相对分子质量大、极性强,难以用色谱方法直接分析,导致目前缺乏能够反映黄芪多糖组成差异的质量评价方法。首先通过部分酸水解方法,将多糖水解成可供分析的寡糖,建立基于部分酸水解-亲水作用色谱的黄芪多糖指纹图谱。通过正交实验选取最佳水解条件:温度80℃、酸浓度1.5 mol/L,水解时间4 h。该方法重复性好,对20批黄芪药材的多糖指纹图谱分析显示相似度为0.258~0.949,反映出黄芪多糖组成的明显差异。同时建立了黄芪的反相液相色谱指纹图谱,用于控制除多糖以外的其他成分,对同样的20批黄芪药材进行分析,实现对黄芪全面的质量评价。实验表明,基于部分酸水解-亲水作用色谱的黄芪多糖指纹图谱可对黄芪多糖的质量进行有效评价,是对黄芪质量评价方法的重要补充。
Polysaccharides is an important component of Astragalus, but the relative molecular mass of polysaccharides, the polarity is strong, it is difficult to direct analysis by chromatographic methods, resulting in the lack of a quality evaluation method that can reflect the differences in the composition of astragalus polysaccharides. Firstly, the polysaccharides were hydrolyzed into oligosaccharides by partial acid hydrolysis to establish the APS fingerprint based on partial acid hydrolysis - hydrophilic interaction chromatography. The optimal hydrolysis conditions were selected by orthogonal experiment: temperature 80 ℃, acid concentration 1.5 mol / L, hydrolysis time 4 h. The repeatability of the method is good, the polysaccharides fingerprinting analysis of 20 batches of Astragalus medicinal herbs shows that the similarity is 0.258 ~ 0.949, which reflects the obvious difference of the composition of astragalus polysaccharides. At the same time, the RP-HPLC fingerprint of Radix Astragali was established to control other components except polysaccharides, and the same 20 batches of Astragalus membranaceus were analyzed to achieve a comprehensive quality evaluation of Radix Astragali. The experimental results show that the APS fingerprint based on partial acid hydrolysis - hydrophilic interaction chromatography can effectively evaluate the quality of Astragalus polysaccharide, which is an important supplement to the quality evaluation of Astragalus membranaceus.