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目的 了解新疆乌鲁木齐地区部分高危人群中庚型肝炎病毒的感染状况。方法 首先运用酶标法(ELISA)检测不同人群中庚型肝炎病毒抗体(抗-HGV),然后对抗-HGV阳性血清运用逆转录聚合酶链反应(RT-PCR)检测HGV RNA。结果 在各类高危人群中,抗-HGV阳性率分别是:手术受血者为35.59％(21/59),静脉吸毒者为38％(19/50),非甲～戊型肝炎病人为18％(9/50);均显著高于对照人群(2％,1/50,P<0.05);职业供血员抗-HGV阳性率也较高(13.79％,8/58),但与对照人群无显著性差异(P>0.05);58份抗-HGV阳性血清有18份血清HGV RNA阳性,其中手术受血者血清1份,静脉吸毒者血清12份,职业供血员血清2份,非甲～戊型肝炎病人血清3份。结论 (1)本研究首次在乌鲁木齐地区部分高危人群中证实有较高的庚型肝炎病毒感染率;(2)再次证实该病毒主要通过注射、输血途径传播。
Objective To understand the prevalence of hepatitis G virus in some high-risk groups in Urumqi, Xinjiang. Methods Antibody-anti-HGV (anti-HGV) in different populations was detected by enzyme-linked immunosorbent assay (ELISA). HGV RNA was detected by reverse transcriptase-polymerase chain reaction (RT- Results The positive rates of anti-HGV were 35.59% (21/59) in the recipients and 38% (19/50) in the intravenous drug users in all kinds of high-risk groups, and 18 % (9/50) in control group were significantly higher than those in control group (2%, 1/50, P <0.05). The positive rate of anti-HGV in professional blood donors was also higher (13.79%, 8/58) (P> 0.05). There were 18 serum HGV RNA positive in 58 anti-HGV positive sera, including 1 serum from the recipients, 12 from the intravenous drug users, 2 from the professional blood donors, ~ Hepatitis E patients serum 3 copies. Conclusions (1) For the first time, this study confirmed a higher prevalence of hepatitis G virus infection in some high-risk groups in Urumqi. (2) It was confirmed again that the virus was mainly transmitted by injection and blood transfusion.