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目的探讨重组人白介素11对放射致肺损伤的保护作用及机制。方法白介素11预先处理小鼠,然后胸腔给予25Gy剂量X线照射。照射后观察动物存活率;用Northern印迹法对肺总RNA样品检测肿瘤坏死因子和过氧化物歧化酶mRNA含量。结果与未用白介素11处理的小鼠相比,白介素11处理的小鼠照射后存活率明显升高,其肿瘤坏死因子mRNA表达受到抑制,而过氧化物歧化酶mRNA表达明显增加。结论白介素11保护放射致肺损伤的分子机制是抑制肿瘤坏死因子和清除活性氧。
Objective To investigate the protective effect and mechanism of recombinant human interleukin 11 on lung injury induced by radiation. Methods Interleukin 11 pre-treated mice and then given a dose of 25Gy X-ray to the chest. The survival rate of the animals was observed after irradiation. The total lung RNA samples were detected by Northern blot to detect the content of tumor necrosis factor and the superoxide dismutase mRNA. Results Compared with the mice not treated with interleukin 11, the survival rate of interleukin 11 treated mice was significantly increased, the expression of tumor necrosis factor mRNA was inhibited and the expression of superoxide dismutase mRNA was significantly increased. Conclusion The molecular mechanism of interleukin 11 in protecting lung from radiation injury is to inhibit tumor necrosis factor and scavenging reactive oxygen species.