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利用太谷显性雄性不育基因Ta1(Ms2)经多亲本杂交、互交、再杂交,建成遗传基础宽阔的原始基础群体,进而开展小麦抗赤霉病性表型轮回选择,建拓基因库.1988/1989和1991/1992年,分别在土表接种和单花接种条件下,对抗源库RC_0-RC_2和RC_1-RC_5轮选世代群体及对照品种进行抗赤霉病性比较试验,以分析群体改良的效果.土表接种的试验结果表明,可育株每穗病小穗数(率)和病粒数(率)明显减少,群体综合抗赤性得到显著提高.RC_0,RC_1和RC_2平均每穗健粒率分别为49.2%,65.5%和70.7%.经两轮选择,群体中病小穗率和病粒率低于30%的植株频率分别提高40%和36%.单花接种条件下的试验结果进一步证明,通过表型轮回选择,抗赤霉病性(主要是抗扩展性)可以得到显著增强,随着基因库的不断建拓,从中选获抗病植株的机会将逐渐增大.从RC_1到RC_5群体平均病小穗数显著减少,平均每轮减少8.6%;病小穗数低于4的抗病植株频率平均每轮提高4.2%;病小穗数大于10的感病植株频率4轮降低9.2%.抗性遗传方差明显变小,遗传变异系数前4轮降低不显著.
Taichuang male sterile gene Ta1 (Ms2) was crossed by multiple parents, crossed and crossed, and the original basic population with broad genetic basis was established. Then the wheat was screened for resistance to scab, In 1988 and 1989 and 1991 and 1992, respectively, against soil-surface inoculation and single-flower inoculation conditions, against the RC source library RC_0-RC_2 and RC_1-RC_5 selected generations of transgenic populations and control varieties against blight test to analyze The results of population improvement showed that the number of spikelets per panicle and the number of disease-free grains per panicle of fertile plants were significantly decreased, and the comprehensive resistance of the population was significantly improved.The average of RC_0, RC_1 and RC_2 The rate of grain-per-panicle was 49.2%, 65.5% and 70.7%, respectively.With two rounds of selection, the frequency of disease with the percentage of diseased spikelets and disease-less than 30% in the population increased by 40% and 36% The results of the experiment further demonstrate that the resistance to scab (primarily spread resistance) can be significantly enhanced by phenotypic recurrent selection and the chances of winning disease-resistant plants will gradually increase as the gene pool continues to grow The average number of spikelets decreased significantly from RC_1 to RC_5, with an average reduction of 8 per round. 6% respectively. The frequency of disease-resistant plants with spikelets less than 4 increased by 4.2% per round on average, and the frequency of susceptible plants with spikelets greater than 10 decreased by 9.2% in 4 rounds. The genetic variance of resistance was significantly decreased, The first 4 rounds of reduction are not significant.