论文部分内容阅读
目的研究骨髓间充质干细胞(BMSCs)子宫局部移植修复宫腔粘连(IUA)子宫内膜损伤的效果。方法本研究模型为IUA新西兰大白兔,使用刮宫和感染双重损伤法建立模型,为实验组(模型组和移植组),将行假手术的新西兰大白兔作为对照组。移植组在建模1周后采用PKH26标记的BMSCs进行局部移植到双侧子宫。成功移植后4周,观察并记录子宫内膜形态变化;计算所有模型的腺体计数和纤维化面积比;采用免疫组化(IHC)对子宫内膜腺上皮的CK和VIM蛋白表达进行检测;采用荧光显微镜定位BMSCs。结果实验组在移植4周后的子宫内膜腺体计数较对照组相比差异有统计学意义(P_(模型)=0.00,P_(移植)=0.01),模型组与移植组比较差异有统计学意义(P=0.00)。对照组的子宫内膜纤维化面积明显大于模型组(P=0.00),而较移植组差异无统计学意义(P=0.53)。各组CK蛋白表达水平比较差异无统计学意义(P=0.17)。VIM蛋白表达情况,模型组与对照组比较差异有统计学意义(P_(模型)=0.00),对照组与移植组比较差异无统计学意义(P=0.92)。移植组在移植4周后的宫颈、阴道及卵巢组织中均可观察到少量红色荧光,其余两组均未发现红色荧光存在。结论 BMSCs子宫局部移植可使IUA新西兰大白兔获得较正常子宫内膜相似的子宫内膜形态学和细胞表型,对临床BMSCs移植治疗IUA有价值。
Objective To study the effect of local transplantation of bone marrow mesenchymal stem cells (BMSCs) in uterus for the repair of intrauterine adhesions (IUA) endometrium. Methods The model of IUA New Zealand white rabbits was established by double-incision with curettage and infection. The experimental group (model group and transplantation group) were sham-operated New Zealand white rabbits as the control group. One week after modeling, PKH26-labeled BMSCs were transplanted to the bilateral uterus. Four weeks after the successful transplantation, the endometrial morphological changes were observed and recorded. The gland count and fibrosis area ratio of all the models were calculated. The expression of CK and VIM protein in endometrial glandular epithelium was detected by immunohistochemistry (IHC) BMSCs were localized by fluorescence microscopy. Results There was significant difference in endometrial gland count between the experimental group and the control group 4 weeks after transplantation (P_ (model) = 0.00, P_ (transplantation) = 0.01). The differences between the model group and the transplantation group were statistically significant Significance of learning (P = 0.00). The area of endometrial fibrosis in the control group was significantly larger than that in the model group (P = 0.00), but there was no significant difference between the two groups (P = 0.53). There was no significant difference in CK protein expression between groups (P = 0.17). VIM protein expression, the difference between the model group and the control group was statistically significant (P_ (model) = 0.00), there was no significant difference between the control group and the transplantation group (P = 0.92). Transplantation group 4 weeks after transplantation in the cervix, vagina and ovarian tissue were observed a small amount of red fluorescence, the other two groups were found no red fluorescence. Conclusion Local transplantation of BMSCs into the uterus can make IUA New Zealand white rabbits obtain endometrial morphology and cell phenotype similar to normal endometrium, which is valuable for clinical treatment of IUA with BMSCs transplantation.