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目的：：分析研究阳春砂基因序列特征，建立基于DNA分子的快速甄别阳春砂的荧光定量PCR技术。方法：收集阳春砂及同科其他样品，经专家鉴别后，提取DNA。根据阳春砂ITS序列两端相对保守区设计引物和探针，优化反应条件，建立阳春砂的荧光定量PCR检测方案。结果：基于阳春砂ITS序列两端相对保守区设计了引物和探针，通过条件优化，建立的荧光定量PCR检测方法能成功对阳春砂样品进行检出，而同科其他非阳春砂样品无扩增曲线。结论：阳春砂药材能够通过除传统专家鉴定方法以外的荧光定量PCR方法快速检出。“,”Objective:To analyze the genetic sequence characteristics of amomum viosum and establish a rapid identification meth-od for amomum viosum by fluorescent quantitative PCR based on DNA analysis. Methods:Amomum viosum and the other samples be-longing to the same genera were collected and identified by experts in the domain. DNA was isolated using commercial kits. The prim-ers and probe were designed according to the conserved region of ITS in amomum viosum. The reaction conditions were optimized to es-tablish the fluorescent quantitative PCR method for the rapid detection of amomum viosum. Results:The fluorescent quantitative PCR method for the rapid detection of amomum viosum was set up. The method could identify amomum viosum successfully, while those samples in the same genera were without amplification curves. Conclusion: Amomum viosum can be identified rapidly by fluorescent quantitative PCR besides the traditional identification by experts.