论文部分内容阅读
目的了解乐平市活禽交易市场禽类禽流感病毒携带及分布情况。方法于2015年4月—2016年12月在乐平市6个活禽交易市场采集市场内笼具涂拭、禽类粪便和饮水等标本210份,采用实时荧光定量聚合酶链反应法(real-time PCR)检测禽流感病毒核酸,并比较不同类型、不同季节采集的标本禽流感病毒阳性率。结果检出禽流感病毒核酸阳性184份,阳性率为87.62%,H5/H9混合型阳性率最高,为32.38%;笼具涂拭阳性率为97.87%,禽类饮水阳性率为100.00%,其他标本阳性率为97.14%,禽类粪便阳性率为80.00%,差异有统计学意义(P<0.05)。鸡粪、鸭粪和鸽粪的阳性率分别为79.17%、89.09%和52.94%,差异有统计学意义(P<0.05)。结论乐平市活禽交易市场环境禽流感病毒核酸检出率高,以H5/H9混合型为主。
Objective To understand the poultry bird flu virus transmission and distribution in live poultry market in Leping. Methods A total of 210 samples of swab, poultry feces and drinking water were collected from 6 live poultry markets in Leping from April 2015 to December 2016. The real-time PCR (real-time PCR) PCR) to detect the avian influenza virus nucleic acid, and to compare the positive rate of the specimens collected from different types and seasons. Results A total of 184 nucleic acid positive samples of avian influenza virus were detected, the positive rate was 87.62%. The positive rate of H5 / H9 mixed type was 32.38%, the positive rate of cage coating was 97.87%, the positive rate of poultry drinking water was 100.00% The positive rate was 97.14%, the positive rate of poultry feces was 80.00%, the difference was statistically significant (P <0.05). The positive rates of chicken manure, duck feces and pigeon were 79.17%, 89.09% and 52.94%, respectively, with significant difference (P <0.05). Conclusion The detection rate of avian influenza virus nucleic acid in live poultry market of Leping City is high, mainly H5 / H9 mixed type.