论文部分内容阅读
螺旋藻经丙酮－水（体积比３∶１）系统在６５°Ｃ浸提３０ｍｉｎ后得到总类脂。总类脂用硝酸银－硅胶柱层析，其中的脂肪酸半乳糖酯被环己烷－乙酸乙酯（体积比２∶８）洗脱出来，蒸去溶剂后，在甲醇－氯乙酰的共同作用下，脂肪酸半乳糖酯转化为脂肪酸甲酯。后者与甲醇和尿素一起于６５°Ｃ回流至澄清，在－１５°Ｃ静置过夜，饱和脂肪酸甲酯和单不饱和脂肪酸甲酯形成尿素复合物结晶析出，γ－亚麻酸甲酯留在母液里面被纯化出来，这种方法可得到纯度为９６．５％的γ－亚麻酸
Spirulina total acetone was obtained after acetone-water (volume ratio 3: 1) system was extracted at 65 ° C for 30 min. The total lipid was purified by silver nitrate-silica gel column chromatography, in which the fatty acid galactosyl ester was eluted with cyclohexane-ethyl acetate (2: 8 by volume). After the solvent was distilled off, , The fatty acid galactosyl ester is converted to fatty acid methyl ester. The latter refluxed to clarify with methanol and urea at 65 ° C and allowed to stand overnight at -15 ° C. The saturated fatty acid methyl ester and the monounsaturated fatty acid methyl ester formed a urea complex and crystallized. The γ-linolenic acid methyl ester remained The mother liquid was purified inside, this method can be obtained with a purity of 96.5% γ-linolenic acid