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目的 探讨血管紧张素ⅡⅠ型受体拮抗剂缬沙坦（ｖａｌｓａｒｔａｎ，Ｖａｌ）在人类肾小管上皮细胞系（ＨＫＣ）转分化中的作用。方法将培养的ＨＫＣ细胞分为（１）无血清培养对照组；（２）阳性对照组（ＭＣＰ－１＋ ＡＡＩ组）：培养液中加入马兜铃酸－Ｉ（ＡＡＩ）和单核细胞趋化蛋白－１（ＭＣＰ－１）；（３）Ｖａｌ组：培养液中加入Ｖａｌ；（４） ＭＣＰ－１＋ ＡＡＩ＋ Ｖａｌ组。应用间接酶标免疫组织化学方法（ＩＥＩ）检测 ＨＫＣ细胞α－平滑肌肌动蛋白（α－ＳＭＡ）、波形蛋白、角蛋白表达的变化，流式细胞技术检测α－ＳＭＡ阳性的ＨＫＣ细胞百分数。结果不同浓度缬沙坦（０．１、１０、１０ ０、１００． ０ ｐｇ／ｍｌ）分别作用于 ＨＫＣ细胞４８ｈ后，ＩＥＩ测定细胞角蛋白、波形蛋白及。ＳＭＡ抗原表达，与无血清对照组比无明显变化；流式细胞术测得Ｖａｌ组表达α－ＳＭＡ阳性的 ＨＫＣ细胞百分数分别为１０． ２％、５．６％、５．２％、０．９％，与无血清对照组（平均为３．１％）比较，差异无显著性意义（ Ｐ＞ ０．０５）。 ＭＣＰ－１＋ ＡＡＩ＋ Ｖａｌ（０．１、 １． ０、１０．０ ｐｇ／ｍｌ）组 ＨＫＣ细胞培养４８ｈ后，表达α－ＳＭＡ阳性的ＨＫＣ细胞分别为１７．?
Objective To investigate the role of angiotensin Ⅱ type 1 receptor antagonist valsartan (Val) in the transdifferentiation of human renal tubular epithelial cell line (HKC). Methods The cultured HKC cells were divided into (1) serum-free control group, (2) positive control group (MCP-1 + AAI group): AAI and monocyte chemotaxis Protein-1 (MCP-1); (3) Val group: adding Val to the culture medium; and (4) MCP-1 + AAI + Val group. The expression of α-smooth muscle actin (α-SMA), vimentin and keratin in HKC cells was detected by indirect enzyme immunohistochemical method (IEI). The percentage of α-SMA positive HKC cells was detected by flow cytometry. Results After different doses of valsartan (0.1, 10, 10, 100, and 100 pg / ml) were administered to HKC cells for 48 hours, the cytokeratin, vimentin and cytokeratin were detected by IEI. SMA antigen expression compared with the serum-free control group had no significant change; flow cytometry measured Val group expressed α-SMA-positive HKC cells were 10%. 2%, 5.6%, 5.2%, 0.9%, no significant difference compared with serum-free control group (average 3.1%) (P> 0.05). After cultured in HKC cells of MCP-1 + AAI + Val (0.1, 1.0, 10.0 pg / ml) group for 48 h, the expression of α-SMA positive HKC cells were 17. ?