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T细胞在自身非T细胞刺激下发生增殖反应称为自身混合淋巴细胞反应(AMLR)。它反映机体免疫系统内的功能调节机制。本文以氚标胸腺嘧啶(~3H-TdR)掺入的淋巴细胞转化试验测定单核细胞(Mon)刺激自身T细胞的增殖反应;用单克隆抗体(McAb)Tu22、Tu36和 Anti-Leu-M,通过间接免疫荧光技术测定 Mon表面 HLA-Ⅱ类抗原的表达,分析急、慢性髓细胞白血病(AML 和CML)患者 AMLR 的改变及其机理。结果表明,与 HLA 全相同的同胞比较,患者 AMLR 明显减弱(p<0.001);患者Tu22~+(HLA-DQ~+)Mon 百分率明显下降(p<0.001),但Tu36~+(HLA-DR~+)Mon 百分率无明显改变(P>0.05)。提示髓细胞白血病患者 AMLR改变与刺激细胞(Mon)表面HLA-DQ 抗原表达障碍有关。
T cell proliferation in their own non-T cell stimulation response known as autologous mixed lymphocyte reaction (AMLR). It reflects the functional regulation of the body’s immune system. In this study, the proliferation of monocytes stimulated by T lymphocytes was measured by lymphocyte transformation assay with tritium thymidine incorporation (~ 3H-TdR). Monoclonal antibodies McAb Tu22, Tu36 and Anti-Leu-M , And the expression of HLA class II antigens on Mon surface was measured by indirect immunofluorescence. The changes and mechanisms of AMLR in patients with acute and chronic myeloid leukemia (AML and CML) were analyzed. The results showed that compared with the same sibling of HLA, the AMLR of patients was significantly decreased (p <0.001); the percentage of Tu22 ~ + (HLA-DQ ~ +) ~ +) Mon percentage had no significant change (P> 0.05). It is suggested that the alteration of AMLR in patients with myeloid leukemia is related to the disorder of HLA-DQ antigen expression on the surface of stimulated cells (Mon).