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目的:通过观察茶多酚联合吉非替尼对人肺腺癌生长的抑制作用,验证茶多酚抗肿瘤血管生成效应。方法:建立人肺腺癌A549移植瘤模型,设立对照组、茶多酚组、吉非替尼组及两者联合组。观察对肺腺癌A549移植瘤的抑制率,检测移植瘤体的微血管密度;并观察EGFR-VEGF这一肿瘤血管生成重要通路的相关因子VEGF、AKT-2、HIF-1α和STAT-3表达水平。结果:茶多酚组、吉非替尼组对移植性人肺腺癌A549瘤体、肿瘤组织微血管密度及VEGF的表达都有明显的抑制效果;两者联合使用效果明显增强;茶多酚组、吉非替尼组能明显抑制AKT-2、HIF-1α和STAT-3表达水平,但两者联合组对AKT-2、HIF-1α作用并不明显,而能明显抑制STAT3表达。结论:茶多酚、吉非替尼对移植性人肺腺癌A549具有明显的抑制效果,两者联合使用有一定增效作用,并能够影响肿瘤血管生成通路的相关因子表达。
Objective: To observe the inhibitory effect of tea polyphenols combined with gefitinib on the growth of human lung adenocarcinoma and verify the anti-angiogenic effect of tea polyphenols. Methods: The human lung adenocarcinoma A549 xenograft model was established. The control group, the tea polyphenols group, the gefitinib group and the combination of the two groups were established. The inhibitory rate of lung adenocarcinoma A549 transplanted tumor was observed and the microvessel density of transplanted tumor was detected. The expressions of VEGF, AKT-2, HIF-1α and STAT-3 were also observed in the tumor angiogenesis pathway of EGFR-VEGF . Results: The tea polyphenols group and the gefitinib group had obvious inhibitory effect on the transplanted human lung adenocarcinoma A549 tumor, the microvessel density of the tumor tissue and the expression of VEGF, and the combined effect of the two was obviously enhanced. The tea polyphenols group Gefitinib could significantly inhibit the expression of AKT-2, HIF-1α and STAT-3, but the combined effect of AKT-2 and HIF-1α was not obvious, but significantly inhibited STAT3 expression. CONCLUSION: Tea polyphenols and gefitinib have significant inhibitory effects on human lung adenocarcinoma A549 transplanted in vitro. The combination of the two shows synergistic effects and can affect the expression of related factors in tumor angiogenic pathway.