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目的建立一种梯度洗脱高效液相色谱法测定蜂王浆中的6种防腐剂。方法参考SN/T 1303—2003出入境检验检疫行业标准—高效液相色谱法,对色谱条件进行优化。结果 6种防腐剂组分分离良好,在0.1~10μg/ml的线性范围内,6组分的线性回归方程均呈良好线性关系,r>0.999,测定低限均达到1 mg/kg,加标回收率范围为95.9%~99.9%。结论该方法灵敏、准确、适用性更强,可满足产品质控的要求。
Objective To establish a gradient elution HPLC method for the determination of six preservatives in royal jelly. Methods Reference to SN / T 1303-2003 entry-exit inspection and quarantine industry standards - high performance liquid chromatography, the chromatographic conditions were optimized. Results Six kinds of preservatives were well separated. The linear regression equation of the six components showed a good linear relationship in the linear range of 0.1 ~ 10μg / ml, r> 0.999, the lower limit of determination was 1 mg / kg, The recoveries ranged from 95.9% to 99.9%. Conclusion The method is sensitive, accurate and adaptable, which can meet the requirements of product quality control.